FEATURED TECHNOLOGY

Advanced Infrastructure for Stem Cell Research

The Stem Cell Core Facility provides state-of-the-art infrastructure to support basic, translational, and regenerative medicine research. Our platform is designed to enable high-quality hiPSC maintenance, gene editing, molecular validation, and advanced imaging within a rigorously controlled environment.

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馃敩 Advanced Imaging & Cellular Characterization

High-resolution imaging platforms support morphological analysis, pluripotency validation, and immunofluorescence studies.

馃抢 Impact: Imaging platforms that directly support high-impact publications.

馃敼 Olympus IX83 Microscope

High-resolution, fully automated observation of live and fixed samples across multiple dimensions (X, Y, Z, and time), supporting stem cell, organoids, embryos, and tissue research.

Key Features

Z-Stack Imaging: Acquire images at multiple focal planes for 3D reconstruction
Automatic Focusing: Multiple objective lenses for precise, reproducible imaging
Time-Lapse Acquisition: Continuous monitoring of dynamic processes in live cultures, organoids, embryos, and organotypic slices
Multimodal Imaging: Bright field, phase contrast, and fluorescence (DAPI, FITC, TRITC)
Fast Image Acquisition & Overlay: View multiple fluorescence channels simultaneously
Multipoint Scanning: Automated imaging at multiple locations for quantitative, reproducible datasets

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馃敼 EVOS M7000 Microscope

Fully automated fluorescence and transmitted-light imaging for live cells, organoids, embryos, tissue slices, and more. Designed for high-content stem cell research, these platforms combine speed, precision, and ease of use.

Key Features

Automated Multichannel Fluorescence Imaging: Rapid, reproducible immunofluorescence data acquisition across multiple channels
High-Quality Optics: 5-position objective turret with 4-color LED fluorescence and transmitted light channels
Dual Camera System: 3.2 MP CMOS color and black/white cameras for bright-field and fluorescence imaging
Automated Imaging: Fully automated X/Y scanning stage, autofocus, exposure control, and objective/light cube switching
High-Speed Acquisition: Z-stack, tile-stitching, 2D/3D deconvolution, and time-lapse imaging for long-term experiments
Environmental Control: Temperature, gas, and humidity regulation for live cell imaging
Advanced Analysis: Optional Celleste鈩� software for cell segmentation, quantification, and visualization
Flexible Workflow Programming: Well-plate scanning, multipoint time-lapse experiments, and montage/area scans in Z-stack or time-lapse modes
Smart Illumination: LED technology ensures consistent fluorescence excitation with minimal phototoxicity
User-Friendly: Intuitive interface for reliable operation across research groups听

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馃敼 Olympus Provi-scanner

Digital whole-slide scanning for histological and immunostained samples.

Key Features & Benefits:

Automated Slide & Culture Scanning: Rapid acquisition of multiple points in culture vessels or slides with minimal hands-on time.
Non-Invasive Live Monitoring: Track cell health, confluency, and growth dynamics without staining or disrupting cultures.
Remote Alerts: Receive notifications when cultures approach confluency and are ready for passage.
High-Resolution Imaging: Bright-field, phase contrast, and fluorescence (DAPI, FITC, TRITC) for publication-quality data.
Multichannel & Z-Stack Imaging: Capture multiple fluorescent markers, tile-stitch large areas, and generate 3D reconstructions.
Data Management & Analysis: Easily store, reuse, transfer, and compare data across experiments and conditions; integrates with downstream analysis software.
Versatile Sample Support: Histological sections, immunofluorescence slides, organoids, and tissue slices.

With fully automated imaging and live culture monitoring, the Olympus Provi-scanner enables quantitative, reproducible, and high-content data collection, empowering researchers to accelerate stem cell research with confidence.

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馃К Gene Editing & Genetic Engineering

Precision Genome Manipulation in hiPSCs, derived-Stem Cells and Primary Cell Cultures.

馃抢 Impact: These systems enable generation of isogenic lines, knockouts, knock-ins, and reporter cell lines.

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馃敼 Neon NxT Electroporation System

鈥淥ptimized for hiPSCs, primary cells, and other hard-to-transfect cell types"

High Transfection Efficiency
Customizable Protocols: Adjustable voltage, pulse width, and number of pulses for precise control
Rapid & Reproducible: Consistent results across experiments and multiple users
Versatile Applications: Supports CRISPR/Cas9 editing, plasmid delivery, RNA transfection, and protein introduction
Minimal Cytotoxicity: Optimized settings reduce cell stress and maintain pluripotency
Multi-User Ready: Reliable platform for academic core facilities and collaborative projects
Seamless Workflow Integration: Compatible with downstream molecular assays, imaging, and functional studies
Accelerates Research: Enables efficient genetic modification to support studies in differentiation, disease modeling, and regenerative medicine

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馃敼 Nucleofector 2b

Proven platform for CRISPR/Cas9 delivery and stable genome editing workflows.

Efficient transfection of hard-to-transfect cell lines and primary cells.
Easy adjustment and configuration of parameters.

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馃И Molecular Characterization & Validation

From DNA to Functional Assays

馃抢 Impact: 听These tools support pluripotency validation, differentiation markers, and genetic screening workflows.

馃敼Real-Time PCR System

QuantStudio 3 Real鈥慣ime PCR System
QuantStudio 5 Real鈥慣ime PCR System

Sensitive and Accurate Quantification: Detect and quantify DNA and RNA from stem cells, hiPSCs, and differentiated derivatives with high precision
Fast & Reliable: Rapid cycling and optimized protocols for time-efficient gene expression and genomic assays
Multiplexing Capability: Simultaneously measure multiple targets using fluorescent probes for complex stem cell pathways
Wide Dynamic Range: Detect low-abundance transcripts, crucial for rare stem cell populations and differentiation studies
Reproducible Results: Standardized workflows ensure consistency across experiments and users
Easy Data Analysis: Integrated software for relative quantification, absolute quantification, and melt curve analysis
Versatile Applications: Gene expression profiling, pluripotency marker validation, CRISPR genotyping, and stem cell differentiation studies
Integration with Core Workflows: Compatible with upstream sample prep (RNA extraction, cDNA synthesis) and downstream validation assays

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馃敼Standard Thermocyclers

ProFlex PCR System
Prime PCR System

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Flexible Thermal Cycling: Multiple blocks and customizable profiles allow simultaneous amplification of different targets, ideal for multi-sample stem cell experiments
High Precision & Accuracy: Uniform temperature control ensures reproducible amplification for DNA, cDNA, and genomic assays
Multiplex Capability: Compatible with multiplex PCR assays for efficient detection of multiple gene targets in stem cells
Rapid Cycling Options: Optimized protocols reduce experiment time without compromising data quality
User-Friendly Interface: Intuitive software simplifies programming, monitoring, and data management
Versatile Applications: Genotyping, pluripotency marker validation, CRISPR validation, and differentiation studies in hiPSCs and other stem cell lines
Integration with Core Workflows: Works seamlessly with RNA/DNA prep, real-time PCR validation, and downstream molecular assays
Reproducible Results Across Users: Ideal for multi-user core facilities requiring consistent and reliable data

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馃敼PCR Hood for DNA/RNA workflows听

Preparation of DNA and RNA samples for PCR, qPCR, and RT-PCR
Setting up gene expression assays and genotyping workflows
Minimizing contamination risk during sensitive nucleic acid handling
Sample preparation for CRISPR validation and molecular cloning
Critical for maintaining data integrity in gene editing, pluripotency validation, and differentiation assays
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馃敼 Lucetta 2 Luminometer

Cell proliferation Assay.
Cytotoxicity Assay.
Luciferase reporter gene assay.
Mycoplasma Detection Assay.
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馃Й Controlled Stem Cell Culture Environment

Reliable & Sterile Culture Infrastructure.

馃抢 Impact: This infrastructure ensures optimal sterility, cell viability, and reproducibility.

Class II Biosafety Cabinets (5)
HERAcell Vios 160i Incubathors
Ultra-Low Temperature (ULT, -80*C) Freezer听
Microcentrifuges
Water & Bead Baths
Electronic Multichannel Pipettes

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馃敼 Ultra鈥慙ow Temperature (ULT) Freezer

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馃敼Microcentrifuges

Separation of cells, supernatants, and subcellular components
Preparation of samples for PCR, RNA/DNA extraction, and enzymatic assays
Pellet formation for cell washing, media changes, and differentiation protocols
Processing of small volumes for downstream analytical assays.
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馃敼Water bath & 听Beads bath
Water Bath
Rapid thawing of cryopreserved cells and cryovials
Warming media, reagents, and supplements
Enzymatic incubations and enzyme activation steps
Temperature-sensitive molecular reaction
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Bead Bath (Dry Bath)
Safe, water-free alternative for thawing cryovials
Warming media and reagents without contamination risk
Incubation of samples in tubes
Supporting standardized molecular and enzymatic workflows.

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馃敼 Electronic Multichannel Pipettes听

Precision, Automation, and High-Throughput Performances.

High-throughput stem cell assays
Standardized differentiation workflows
CRISPR screening plate preparation
Molecular assay reproducibility

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馃К Specialized hiPSC or derived-Stem Cells & WorkflowSupport

"Dedicated infrastructure to ensure optimal viability, morphology control, and reproducibility in stem cells workflows"

馃敼 Sterile Dissection Hood + Olympus CKX53

For Colony picking/cleaning, stem cells picking/cleaning & morphology monitoring听

4x, 10x, 20x, and 40x magnification.
Bright field,听
Phase contrast,听
Fluorescence (DAPI, FITC, TRITC).
Quickly acquisition and overlapping of images to view samples with multiple fluorescent lenses.听

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馃敼 Automated Cell Counters:

Countess II FL
Countess 3

Highly accurate and precise brightfield and fluorescence cell counting.
Clump cell count.
Automatic lighting and focus adjustment for optimal contrast and resolution.
Automatic saving of cell counts and data.
The software includes convenient built-in pre-dilution and cell-splitting calculators, especially for stem cell culture work.
Time to count two samples (sides A and B) ~1 minute
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馃敼 Sterilization Hood (UVP) + "Cell Culture Marker"听

馃敼 Automated Cell Thawing System (ThawSTAR CFT2)听

鉃� Standardized defrosting
鉃� Reduces variability. Reproducible thawing profiles using a standardized thawing method.
鉃� Ideal for cell banks

鉃� Eliminate the risk of contamination

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馃Й 听Controlled Stem Cell Culture Environment

Equipment that supports cell maintenance, functional assays, and experimental stability.

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馃敼 ThermoMixer C (Interchangeable Thermoblock 鈥� Eppendorf)

** SmartBlocks for 15 ml tubes and plates

鉃� Sample preparation
鉃� Short incubations
鉃� Enzyme assays

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馃敼 MicroPlate Centrifuge

鉃� Rapid plate centrifugation
鉃� ELISA, plate assays, PCR plates

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馃敼 CO2 Orbital Shaker Incubator

鉃� Suspension cultures
鉃� Organoids
鉃� Differentiation under shaking.

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馃敼 Microsample Incubator

鉃� Controlled short incubations
鉃� Rapid assays

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馃敼 3D Orbital Mixer

鉃� Gentle sample mixing
鉃� Differentiation protocols

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Imaging Support Platforms/ Training Platforms

馃敼 Evos XL Core Microscopes

鉃� Routine Microscopy. Objectives at 4x, 10x, 20x, and 40x.
鉃� Daily Morphological Assessment

鉃补蝉测 image acquisition and storage during cell culture work.
鉃� User Training

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Biosafety cabinets with or without lab supplies and consumables.

For questions about lab equipment,听please听contact the Center!

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Videos:听

Olympus IX83 Microscope

IX83: Trans-mesenteric Migration of Enteric Neural Crest Derived Cells (ENCCs)听
IX83: Brain Slice Culture Glia Cell听
听IX83: Real-time Imaging for Ring1BYFP/YFP ME听
听IX83: Synthetic ERK Activity Propagation by a Light-Switchable System听
听IX83: Light-induced Oscillatory/Sustained Expression of Ascl1 in Neural Progenitor Cells
IX83 Inverted Microscope: Adding Oil to the Objective听
IX83 Inverted Microscope: Loading a Sample Slide on the Stage
IX83 Inverted Microscope: Focusing and Positioning the Stage Using the U-MCZ Remote Controller

Neon NxT Electroporation System

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抖阴旅行射

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