Specialized Equipment

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EVOS M7000 Microscope

IIIA

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samples

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  • Outstanding image quality and versatility with a 5-position objective turret, 4-color LED fluorescence and transmitted light channels, and 3.2 MP CMOS color and B/W cameras.
  • Fully automated X/Y scanning stage, autofocus, and acquisition routines.
  • High-speed image acquisition (scanning, Z-stack, and tile-stitch,Ìý2D/3D- deconvolution, visualization, and analysis).
  • Time-lapse live cell imaging (control of temperature, gases, and humidity).
  • Image analysis (cell segmentation and quantification with the optional Cellesteâ„¢ Image Analysis Software).
  • High-quality automated fluorescence imaging.
  • Full automation of the precision X/Y-stage movement; changing of the 4 LED fluorescent light cubes, 5-position objective turret, focus, and exposure; and switching of the dual camera.
  • Can program to run well-plate scanning, time-lapse experiments, and tile-stitch/montage area scans in Z-stack and/or time-lapse modes in your vessel of choice.
  • Versatile and highly configurable with a 5-position objective turret and the ability to simultaneously acquire images in four fluorescence channels plus transmitted light.
  • An extensive suite of tools for acquisition, visualization, and analysis.
  • Smart LED illumination technology.
  • Easy to use and reliable!

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Olympus IX83 Microscope

Olympus IX83
samples olympus 83
  • ÌýFully automated multidimensional observation (X, Y, Z, and time) of live and fixed cells, organoids, embryos, organotypic slices, tissues, etc.
  • Z-stack images.
  • Automatic focusing with multiple objective lenses.ÌýTime-lapse acquisitions can easily be created for high-magnification and multipoint observation.
  • Bright field, phase contrast, or fluorescence (DAPI, FITC, TRITC).
  • Fast image acquisition and overlay to view samples with multiple fluorescent lenses.
  • Automatically scanning multiple points at regular time intervals provides a steady stream of quantitative data in live cultures and organotypic slices.

Videos: Ìý

  • IX83: Trans-mesenteric Migration of Enteric Neural Crest Derived Cells (ENCCs)Ìý
  • IX83: Brain Slice Culture Glia CellÌý
  • ÌýIX83: Real-time Imaging for Ring1BYFP/YFP MEÌý
  • ÌýIX83: Synthetic ERK Activity Propagation by a Light-Switchable SystemÌý
  • ÌýIX83: Light-induced Oscillatory/Sustained Expression of Ascl1 in Neural Progenitor Cells
  • IX83 Inverted Microscope: Adding Oil to the ObjectiveÌý
  • IX83 Inverted Microscope: Loading a Sample Slide on the Stage
  • IX83 Inverted Microscope: Focusing and Positioning the Stage Using the U-MCZ Remote Controller

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Sterile Dissection Hood : Picking hiPSCs/Stem cells Hood + Olympus CKX53

Olympus CKX53
  • Phase-contrast.
  • 4x, 10x, 20x, and 40x magnification.
  • Bright field, phase contrast, or fluorescence (DAPI, FITC, TRITC).
  • Quickly acquisition and overlapping of images to view samples with multiple fluorescent lenses.Ìý

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Olympus Provi-scanner

Olympus Provi
  • ÌýAutomatically scanning multiple points at regular time intervals in culture vessels provides a steady stream of quantitative data about the health and confluency of cultures remotely.
  • No need toÌýstain or peel the cell cultures to check cell growthÌýstatus.
  • Remotely alert when the culture approach confluency and is ready for passage.
  • Easy storage, reuse, transfer of data, comparison with past results, or data captured under different conditions.

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Neon NxT Electroporation System

neon
  • ÌýTransfect even the most challenging cell types with high efficiency, cell recovery/viability, and reproducibility.
  • Transfect cells quickly with an easy three-step workflow, a single buffer kit, and an intuitive user interface.
  • Precisely optimize electroporation parameters for different cell types, cell densities, payloads, and electroporation protocol.
  • Minimize sample transfer loss and contamination risk.
  • Video:Ìýhttps://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/neon-electroporation-system.html?ef_id=Cj0KCQjw6cKiBhD5ARIsAKXUdyY2g2bL7CucWvZRKTsCWMSO7X7p_nt3mjvIpXoPCkueyPdtlvpTx1QaAvUkEALw_wcB:G:s&s_kwcid=AL!3652!3!652579792520!e!!g!!neon%20electroporation!2058097106!75756882203&cid=bid_clb_tfx_r01_co_cp0000_pjt0000_bid00000_0se_gaw_bt_pur_con&gclid=Cj0KCQjw6cKiBhD5ARIsAKXUdyY2g2bL7CucWvZRKTsCWMSO7X7p_nt3mjvIpXoPCkueyPdtlvpTx1QaAvUkEALw_wcB

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Nucleofector 2b Transfection System

NUCLEOFE CTOR

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  • Efficient transfection of hard-to-transfect cell lines and primary cells.
  • Easy adjustment and configuration of parameters.

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Automated Cell Counters: Countess II FL and Countess 3

ACC
  • Highly accurate and precise brightfield and fluorescence cell counting.
  • Clump cell count.
  • Automatic lighting and focus adjustment for optimal contrast and resolution.
  • Automatic saving of cell counts and data.
  • The software includes convenient built-in pre-dilution and cell-splitting calculators, especially for stem cell culture work.
  • Time to count two samples (sides A and B) ~1 minute

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Incubator Orbital Shaker CO2

SHAKER 1
  • ÌýOrganoid growth.
  • Permanent conservation inside an incubator, which allows easy access and assembly.
  • Easy adjustment and configuration of RPM and time.
  • Plates, dishes, and flasks stick to the surface, even when quickly shaken.

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Thermocyclers

  • ProFlex PCR System
  • Prime PCR System
PCR system

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pcr

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Real‑Time PCR System

  • QuantStudioâ„¢ 3 Real‑Time PCR System
  • ABI 7500 Real‑Time PCR System
  • RTqPCR

UV Sterilizing cabinet

Cell Culture Marker + Sterilization Hood (UVP)

UV
Cell Marker Objective

Dead Air Box

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EvosXL Core Microscopes

EVOS
  • ÌýQuick and easy observation of cell cultures without the need to use eyepieces.
  • Objectives at 4x, 10x, 20x, and 40x.
  • Easy change of the aperture to adjust the desired viewing conditions.
  • High-quality LED display.
  • Easy image acquisition and storage during cell culture work.

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Lucetta 2 Luminometer

Luminometer
  • Cell proliferation Assay.
  • Cytotoxicity Assay.
  • Luciferase reporter gene assay.
  • Mycoplasma Detection Assay.

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ThawSTAR® CFT2 Automated Cell Thawing System

THAWING
  • Thawing Time: Less than three minutes
  • Obtain reproducible thawing profiles using a standardized thawing method.
  • Eliminate the risk of contamination from water bath-based thawing techniques.

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E1-ClipTipÌýBluetoothÌýElectronic Multichannel Pipettes

multichannel pipettes
  • Enhanced accuracy and precision.ÌýAutomate daily 96-and 384-well microplate pipetting
  • Two operating options (step-based Matrix or Presets with settings for most common applications)
  • Storing 30 of theÌýmost common Matrix or Presets protocols with a specific name.
  • Customize theÌýmain menu view by organizing the icons ofÌýpreferred order or creating favorite programs and pipetting functions.
  • Connect theÌýpipette to the My Pipette Creator app conveniently via Bluetooth**
  • Choose aÌývolume range from 0.5μL to 1250μL:
    • Single channels
    • Multichannel with 8, 12, or 16 channels in 96 and 384 formats
    • Adjustable tip spacing multichannel with 6, 8, and 12 channels in 96 and 384 formats.

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Thermo Fisher Scientific HERAcell Vios 160i incubators

  • ÌýRemote temperature and CO2 levels monitoring ensureÌýan optimal culture environment for cell cultures (normoxic and hypoxic conditions).
  • Copper interiors provide an antimicrobial surface to avoid contamination and cell death.
  • Normoxic or Hypoxic Conditions.

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Water bath and bead bath

water bath

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beads bath

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Ultra‑low temperature (ULT) freezer

-80

Biosafety cabinets with or without lab supplies and someÌýconsumables.

For questions about lab equipment,ÌýpleaseÌýcontact the Center!